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1.
Vet Res Commun ; 2024 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-38656656

RESUMO

Coxiella burnetii is the etiologic agent of Q fever, a worldwide zoonosis. Cattle, sheep and goats are considered the main reservoirs of the disease. Transmission to humans occurs mainly through the inhalation of infectious aerosols from milk, faeces, urine, and birth products from infected ruminants. In this study, a 2-year longitudinal approach was performed to ascertain the excretion of C. burnetii in bulk tank milk samples of sheep from a mountain plateau in central Portugal, with sampling conducted during the years 2015 and 2016. From a total of 156 bulk tank milk samples tested by qPCR, only one showed to be positive for C. burnetii (1.28% [95%CI: 0.03-6.94]), from 2015, the first year of collection. Bidirectional sequencing and phylogenetic analysis of IS1111 transposase partial region confirmed the presence of C. burnetii DNA. The presence of C. burnetii in raw milk samples highlights the necessity for additional research to determine if raw milk is a potential source for human infection. Animal health surveillance and prevention measures against this zoonotic disease should be considered.

2.
Animals (Basel) ; 14(8)2024 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-38672389

RESUMO

The production of small ruminant autochthonous breeds in the Centre region of Portugal is practiced in a semi-extensive husbandry system, exposing animals to parasitic infections. The main objective of this study was to estimate the prevalence of lungworm infection and identify risk factors. Fecal samples of 203 goats and 208 sheep from 30 herds were collected per rectum and subjected to the modified Baermann test. The overall prevalence of infection was 57.7%, significantly higher in goats (95.6%) than in sheep (20.7%) (p < 0.001). According to the binary logistic regression model, sheep dewormed with albendazole, mebendazole plus closantel, or ivermectin plus clorsulon presented a risk of Protostrongylidae infection 29.702, 7.426, or 8.720 times higher, respectively, than those dewormed with eprinomectin. Additionally, the presence of gastrointestinal parasites was investigated in 307 fecal samples using Mini-FLOTAC®. The overall prevalence of infection was 86.3%, also significantly higher in goats (93.2%) than in sheep (79.9%) (p < 0.001). Strongyle-type eggs were the most frequently identified, both in sheep (69.8%) and goats (87.8%), followed by Eimeria oocysts (40.3% in sheep and 68.9% in goats). Considering the high prevalence and the burden of lungworm parasitic infection, it is urgent to determine its economic impact and the repercussions in animal health in the Centre region of Portugal to establish appropriate therapeutic guidelines.

3.
Pathogens ; 11(10)2022 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-36297274

RESUMO

(1) Background: Toxoplasma gondii is a widespread zoonotic agent that greatly impacts Public Health, being responsible for one of the most important parasitic zoonosis worldwide. T. gondii has a heteroxenous life cycle, with cats being the definitive hosts and all warm-blooded animals, including humans, being intermediate hosts. Veterinary practitioners (VP) may be at a higher risk than the general population for T. gondii infection, as they have direct and daily contact with many animal species. The aim of the present study was to ascertain if VP were more likely to be anti-T. gondii IgG seropositive than the general population, as well as to understand if age, accidents with blood-contaminated sharps (cross-blood contamination), gender, working years, and geographic regions play a role as risk factors for T. gondii infection. For this purpose, a case−control study using archived samples was performed. (2) Methods: A total of 350 veterinary practitioners were tested using a commercial semiquantitative enzyme immunoassay for anti-T. gondii IgG. From the general population, 175 anonymous volunteers (matched with cases by region, age, and gender) were studied for anti-T. gondii IgG. (3) Results: There was no statistical difference found between the presence of anti-T. gondii IgG in practitioners (26%; CI = 21.40−30.60%) and the general population (33.14%; CI = 26.17−40.12%) (p = 0.108). Univariate and multivariate analysis showed that only age (older groups) was found to be associated with a higher prevalence of anti-T. gondii IgG, with significant p values (p < 0.05) for both univariate and multivariate analysis. (4) Conclusions: To the best of our knowledge, this is the first case−control study fully focused on the prevalence of anti-T. gondii IgG in VP in Portugal, showing that there was no significant risk for T. gondii infection in veterinarians exposed daily and repeatedly to different species of animals.

4.
Trop Anim Health Prod ; 54(4): 237, 2022 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-35864408

RESUMO

Crimean-Congo hemorrhagic fever virus (CCHFV) is a widespread zoonotic pathogen that can cause mild to severe hemorrhagic disease in humans. CCHFV may be transmitted through direct contact with tissue or blood of viremic animals; however, the primary transmission route is through infected tick bites. CCHFV RNA has been detected in ticks feeding on domestic and wild animals in western Spain, suggesting an established circulation of CCHFV in Western Europe. Ruminants have been recognized as important CCHFV reservoirs and have been linked to human cases in endemic regions. Given the emergence of CCHF in neighboring Spain, and a report of two CCHFV seropositive humans in southern Portugal in 1985, we investigated the potential circulation of this virus in the country by performing a nationwide anti-CCHFV IgG serosurvey in sentinel sheep of Portugal. Sera (n = 459) randomly selected from widely distributed farms (n = 20) of Portugal were tested using a commercial double-antigen enzyme-linked immunosorbent assay, yielding an overall seroprevalence of 0.4% (95% confidence interval [CI] 0.04-1.56%). Positive sheep were from the southern region of Portugal (Alentejo region), which raise the seroprevalence of this region to 0.74% (95% CI 0.09-2.66%). This is the first study reporting the presence of CCHFV antibodies in sheep of Portugal, thus suggesting a geographical expansion of CCHFV to this country. It seems likely that CCHFV may exist focally in southern Portugal.


Assuntos
Vírus da Febre Hemorrágica da Crimeia-Congo , Febre Hemorrágica da Crimeia , Doenças dos Ovinos , Carrapatos , Animais , Anticorpos Antivirais , Vírus da Febre Hemorrágica da Crimeia-Congo/genética , Febre Hemorrágica da Crimeia/epidemiologia , Febre Hemorrágica da Crimeia/veterinária , Humanos , Portugal/epidemiologia , Estudos Soroepidemiológicos , Ovinos , Doenças dos Ovinos/epidemiologia
5.
Trop Anim Health Prod ; 54(4): 199, 2022 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-35668327

RESUMO

Rickettsiosis is considered an emerging/re-emerging vector-borne disease that causes significant public health threats. Ticks are reservoirs and vectors of Rickettsia having a significant role in the transmission of rickettsiae. In Portugal, little is known about tick-borne Rickettsia species in sheep. Therefore, this study aimed to investigate rickettsiae infection in ticks and their sheep host from 27 farms in four districts of central Portugal, to clarify the role of the sheep host in the circulation of this zoonotic agent. Between March and May 2021, EDTA blood samples (n = 100) of healthy grazing sheep and their ticks (n = 100, one tick per animal) were collected during a herd health program in central Portugal. Obtained ticks were identified as Rhipicephalus sanguineus sensu lato by PCR targeting a partial sequence of 16S rRNA gene followed by sequence analysis. Rhipicephalus sanguineus s.l. and host sheep blood were tested for the presence of Rickettsia spp. by PCR targeting a partial sequence of ompB and ompA genes. From a total of 100 paired R. sanguineus s.l. and host sheep, Rickettsia massiliae was detected in 62 ticks and 35 grazing sheep blood samples, collected in central Portugal, 2021. All 35 positive sheep had attached positive R. sanguineus s.l., with matching nucleotidic sequences. These findings suggest that sheep may develop rickettsiemia and are likely capable of transmitting and amplifying the infection to uninfected ticks maintaining rickettsiae in circulation in the domestic cycle.


Assuntos
Rhipicephalus sanguineus , Infecções por Rickettsia , Rickettsia , Doenças dos Ovinos , Animais , Portugal , RNA Ribossômico 16S/genética , Rhipicephalus sanguineus/genética , Rhipicephalus sanguineus/microbiologia , Rickettsia/genética , Infecções por Rickettsia/epidemiologia , Infecções por Rickettsia/microbiologia , Infecções por Rickettsia/veterinária , Ovinos/genética , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/microbiologia
6.
Pathogens ; 11(5)2022 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-35631124

RESUMO

Toxoplasmosis is a worldwide zoonotic disease with different and complex routes for transmission. Workers occupationally exposed to animals or raw meat and viscera (WOE) may be at more risk than the general population, however conflicting data exist on the risk of developing toxoplasmosis due to this close contact. To add knowledge to this topic, the aim of the present study was to ascertain if WOE were more likely to be anti-T. gondii IgG seropositive than the general population as well as to study risk factors for T. gondii infection such as professional activity, gender, age, years of work and region. For this purpose, a case−control study using archived samples was setup. A total of 114 WOE (including pig slaughterhouse workers, butchers, veterinarians and farmers) and 228 anonymous volunteers (matched with cases by region, age and gender) were studied for anti-T. gondii IgG. A significantly higher anti-T. gondii IgG occurrence (p = 0.0282) was found in WOE when compared with the general population (72.8% [CI = 64.6−81.0%] versus 60.1% [CI = 54.6−65.6%]). Multivariate analysis showed that WOE of more than 50 years of age were more likely to be seropositive for anti-T. gondii IgG (aOR = 16.8; 95% CI 3.6−77.5; p < 0.001) than those aged less than 50 years. To our knowledge, this is the first case−control study on the prevalence of anti-T. gondii IgG in WOE in Portugal, also showing an added risk for T. gondii infection in those exposed to animals or their meat and viscera.

7.
Animals (Basel) ; 12(6)2022 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-35327128

RESUMO

Canine Leishmaniosis (CanL) is a chronic and potentially fatal disease. In economically disadvantaged regions, costs associated with long-term patient monitoring may determine that some owners decline veterinary follow-up of their dogs. This online, questionnaire-based survey aimed to assess how Portuguese veterinary practitioners perform long-term patient monitoring and recognize relapses. More than 50% of respondents reported that 50-100% of dog owners declared financial restraints. Hence, in these circumstances, most veterinary practitioners only performed clinical examination and serology. However, when owners did not declare financial restriction, other tests were additionally performed, such as renal and hepatic profiles, hemogram, serum protein electrophoresis and urine protein creatinine ratio. The mean number of exams performed when owners presented financial restraints was significantly lower than the number of exams performed without economic limitations. Most veterinary practitioners prescribed allopurinol ad aeternum or until disease remission and domperidone. CanL relapses were recognized by more than half of respondents "Always", through the reappearance or worsening of clinical signs, whereas about a quarter detected an increase in anti-Leishmania antibody levels and identified abnormalities in the serum protein electrophoresis profile. The relapse rate was higher in the Lisbon Metropolitan Area and north, the most economically favored regions of Portugal. This study confirms that owner financial restraints negatively influence veterinary follow-up and relapse recognition, ultimately compromising clinical decision making and favoring the maintenance of Leishmania infantum infection endemicity.

8.
Vascular ; 30(4): 803-808, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34144651

RESUMO

OBJECTIVES: This study was designed for evaluation of CEUS (contrast-enhanced ultrasound) for the detection of endoleaks after EVAR (endovascular aortic aneurysms repair) as an alternative to CTA (computed tomography angiography), the gold standard in post-EVAR surveillance. METHODS: Post-EVAR surveillance of patients who underwent CEUS and CTA was retrospectively analyzed to compare the accuracy of CEUS compared to CTA. For that, the following parameters were analyzed: the largest aneurysm diameter, type of endoleaks, and the time elapsed after EVAR using both surveillance tests. RESULTS: The study involved 110 pairs of exams in patients with infrarenal aortoiliac or isolated iliac artery aneurysm, covering predominantly a male population (89%). The time elapsed after EVAR using CEUS or CTA exams were statistically similar, ranging from one to 58 months (mean 12.2) and one to 65 months (mean 9.7), respectively (p = 0.124). CEUS sensitivity was 75.5%, specificity 96.7%, false positives were 24.5%, and false negatives were 3.3%. The accuracy between the two exams was 87.3%. A secondary analysis, comparing CTA with CEUS as a reference standard, revealed CEUS sensitivity of 24.5%, higher than CTA for detecting endoleaks, with a concordance rate of true positive results of 75.5%. Among the endoleaks detected solely by CEUS (12 cases), one case was type Ia and eleven were type II, while those detected only by CTA (2 cases), one was type Ia and one type II. Additionally, a type II endoleak associated with type Ib, identified by CEUS, was seen as type II for CTA only. There was no difference between the pre-EVAR and the post-EVAR diameters of aortoiliac aneurysm (p = 0.058), both for CEUS and CTA. Computed tomography angiography, on the other hand, showed significant aneurysm diameter reduction compared to CEUS for isolated iliac artery aneurysms (p < 0.001). CONCLUSION: Contrast-enhanced ultrasound was more effective than CTA in identifying and characterizing endoleaks in patients undergoing EVAR, especially type II endoleaks. The advantages include efficacy and, particularly, safety, and must be considered in EVAR surveillance protocols so that its use becomes widespread. We understand that CEUS, as a surveillance exam, considerably reduces risks to patients compared to CTA.


Assuntos
Aneurisma da Aorta Abdominal , Implante de Prótese Vascular , Procedimentos Endovasculares , Aneurisma da Aorta Abdominal/diagnóstico por imagem , Aneurisma da Aorta Abdominal/etiologia , Aneurisma da Aorta Abdominal/cirurgia , Aortografia/efeitos adversos , Aortografia/métodos , Implante de Prótese Vascular/efeitos adversos , Angiografia por Tomografia Computadorizada/efeitos adversos , Meios de Contraste/efeitos adversos , Endoleak/diagnóstico por imagem , Endoleak/etiologia , Procedimentos Endovasculares/efeitos adversos , Humanos , Masculino , Estudos Retrospectivos , Resultado do Tratamento
9.
Animals (Basel) ; 11(12)2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34944123

RESUMO

Fasciola hepatica is a parasite that is widespread in Europe, having been reported in ruminants of several European countries and causing an important economic impact. This study ascertained the occurrence and distribution of fasciolosis in Portuguese ovine livestock by assessing F. hepatica IgG antibodies in a cohort of confined sheep from a high-altitude region of central Portugal in a 2-year period. Positive animals were found in most locations and in both years, with 18 of the 92 animals (19.6% [95% confidence interval CI: 12.03-19.15]) and 17 of the same 92 animals (18.5% [95% CI: 11.15-27.93]) showing to be seropositive in the first year and second year, respectively (p = 0.85). Pasture contamination by F. hepatica eggs could be reduced by thorough anthelmintic treatments.

10.
Animals (Basel) ; 11(9)2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34573591

RESUMO

Weed control in vineyards demands regular interventions that currently consist of the use of machinery, such as plows and brush-cutters, and the application of herbicides. These methods have several drawbacks, including cost, chemical pollution, and the emission of greenhouse gases. The use of animals to weed vineyards, usually ovines, is an ancestral, environmentally friendly, and sustainable practice that was abandoned because of the scarcity and cost of shepherds, which were essential for preventing animals from damaging the vines and grapes. The SheepIT project was developed to automate the role of human shepherds, by monitoring and conditioning the behaviour of grazing animals. Additionally, the data collected in real-time can be used for improving the efficiency of the whole process, e.g., by detecting abnormal situations such as health conditions or attacks and manage the weeding areas. This paper presents a comprehensive set of field-test results, obtained with the SheepIT infrastructure, addressing several dimensions, from the animals' well-being and their impact on the cultures, to technical aspects, such as system autonomy. The results show that the core objectives of the project have been attained and that it is feasible to use this system, at an industrial scale, in vineyards.

11.
Pathogens ; 10(1)2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33419095

RESUMO

(1) Background: Toxoplasma gondii is an important zoonosis and one of the major causes of abortion in sheep worldwide. (2) Methods: We performed a 2-year longitudinal serological anti-T. gondii IgG screening on a cohort of a spatially confined population of a Portuguese autochthonous sheep breed in central Portugal. (3) Results: From the screening of the 2015 and 2016 sera, an increase of seroprevalence was observed (57.7% (95% CI: 49.9-65.3%) versus 69.1% (95% CI: 61.5-75.9), from 2015 and 2016, respectively) (p = 0.031). (4) Conclusions The present study is the first to provide prospective data on the anti-T. gondii serological status of a sheep cohort in Portugal, showing an increase in the occurrence of T. gondii. There is a need to provide a clearer understanding of T. gondii epidemiology in Portugal, ideally by implementing monitoring programs on sentinel herds, not only due to the high impact of T. gondii on animal health but also for it being a zoonosis.

12.
Ecohealth ; 17(2): 255-257, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32651733

RESUMO

Q fever is a zoonosis caused by Coxiella burnetii, and transmission to humans is often associated with contact with ovine and caprine livestock. Those exposed to sheep are particularly at high risk of infection. Recent studies show that Q fever is increasing in sheep farms in Portugal raising alerts on spillover to humans. We detected anti-C. burnetii IgG in shepherds and sheep milk cheesemakers (27 [28.1%] in a total of 96; 95% confidence interval [CI] 19.4-38.2%) and in controls (21 [8.1%] in a total of 260; 95% CI 5.1-12.1%), pointing to an increased risk of C. burnetii infection (P = 0.0001), with an odds ratio for anti-C. burnetii of 4.45 (95% CI 2.4-8.4%; P = 0.0001), in individuals with occupational contact with sheep in Portugal.


Assuntos
Fazendeiros/estatística & dados numéricos , Doenças Profissionais/epidemiologia , Febre Q/epidemiologia , Zoonoses/epidemiologia , Animais , Indústria de Processamento de Alimentos , Humanos , Exposição Ocupacional/estatística & dados numéricos , Portugal , Ovinos
13.
Transbound Emerg Dis ; 67(5): 1918-1921, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32090484

RESUMO

Hepatitis E virus (HEV) is an enteric RNA virus from the family Hepeviridae with five genotypes (genotypes 1-4 and 7) known to infect humans. HEV infection is known to have a zoonotic swine origin in industrialized countries. The role of pigs and wild boars as major reservoirs for human infection is today well-established; however, the list of new animal reservoirs is ever-expanding as new HEV strains are continuously being found in a broad host range. The recent detection of HEV in sheep stools brings concerns on the possibility of HEV transmission from these animals to humans, particularly in those occupationally exposed. The present work investigated the potential occupational risk of HEV infection in shepherds and sheep milk cheesemakers-workers occupationally exposed to ovine (WOEOs; N = 96)-from a region of the Centre of Portugal ('Serra da Estrela') based on the differences of anti-HEV IgG seroprevalence rates between these professionals and the general population (N = 192). The presence of HEV-specific antibodies in sheep (N = 90) from the same region was also evaluated. The HEV seroprevalence in WOEOs (29.3%) was found to be significantly higher (p = .0198) when compared with population controls (16.1%) which suggests an increased risk for HEV infection in these workers. HEV-specific antibodies were also found in 16.6% of the studied sheep showing that HEV circulates in these animals. Further studies are needed to confirm the zoonotic potential of sheep HEV.

14.
Vector Borne Zoonotic Dis ; 19(9): 708-710, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-30990772

RESUMO

Introduction: In 2011, Schmallenberg virus (SBV) was first detected in dairy cattle herds in The Netherlands and Germany having since then spread across Europe. Today studies are starting to show a decrease in new SBV infections, a circumstance that raises alerts for possible re-emergence if ideal conditions for vector development occur. To assess the potential decrease in SBV circulation, we performed a 2-year longitudinal serological investigation for SBV infection at the herd level by using bulk-tank milk of a specific sheep breed from central Portugal. Materials and Methods: Bulk-tank milk samples from 68 flocks were collected in both 2015 and 2016, and lactosera were tested for IgG anti-SBV by EIA. Results and Discussion: Results show that in 2015, 92.6% (95% confidence interval [CI]: 83.9-96.8) of the bulk-tank milk samples were positive, whereas in 2016 only 77.9% (95% CI: 66.7-86.1 of the samples from the same flocks were positive. Differences in the 2015/2016 seroprevalences showed to be statistically significant (p = 0.027). This significant decrease at the herd level seems to be in agreement with reported data from other European countries and raise alerts, since increasingly favorable conditions (higher number of susceptible animals) are now present, potentially favoring SBV epidemics if improved conditions for midge replication occur in the future.


Assuntos
Infecções por Bunyaviridae/veterinária , Leite , Orthobunyavirus/isolamento & purificação , Doenças dos Ovinos/virologia , Animais , Infecções por Bunyaviridae/epidemiologia , Infecções por Bunyaviridae/virologia , Portugal , Estudos Soroepidemiológicos , Ovinos , Doenças dos Ovinos/epidemiologia
15.
Ecohealth ; 15(4): 871-874, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30255415

RESUMO

Q fever is a zoonotic disease caused by Coxiella burnetii that is highly prevalent across the world. In this study, a prospective serosurvey was performed to study C. burnetii circulation in a population of sheep in the central region of Portugal. Blood from a representative sample of 168 animals was drawn in both 2015 and 2016, and sera were tested for IgG anti-C. burnetii by EIA. In 2015, 7.7% (13/168) animals tested positive for IgG anti-C. burnetii, while in 2016, 17.3% (29/168) tested positive, showing a statistically significant (P = 0.008) increase in anti-C. burnetii seroprevalence. Results support the notion that Q fever is emerging in central Portugal.


Assuntos
Coxiella burnetii/isolamento & purificação , Febre Q/veterinária , Doenças dos Ovinos/diagnóstico , Animais , Portugal , Estudos Soroepidemiológicos , Ovinos
16.
Vector Borne Zoonotic Dis ; 18(11): 601-604, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-29969389

RESUMO

INTRODUCTION: Q fever is an almost global zoonotic disease caused by Coxiella burnetii. Human infections can produce acute and chronic disease that can lead to abortions and stillbirths in pregnant women, usually infected by the inhalation of C. burnetii-contaminated aerosols or through consumption of contaminated products. Sheep are one of the primary animal reservoirs with disease being associated with vast shedding of bacteria in placentas, feces, milk, and birth fluids. Although almost neglected in the past, recent outbreaks of sheep origin have alerted the public and the scientific community. MATERIALS AND METHODS: An epidemiologic survey to estimate the seroprevalence of Q fever antibodies was performed in a representative number of sheep of all regions of continental Portugal (n = 1068), using a commercial ELISA (ID Screen Q Fever Indirect Multi-species Kit; IDvet™, Montpellier, France). RESULTS AND DISCUSSION: An anti-C. burnetii seroprevalence of 11.4% (95% confidence interval 9.6-13.5) was found, with a clear distinction between the Center region with highest seroprevalence, and the rest of the territory. Sheep traditional farming is widely present in Portugal and is part of the cultural and gastronomical background of the country. This close proximity to small ruminants may contribute to the zoonotic transfer to humans.


Assuntos
Anticorpos Antibacterianos/sangue , Coxiella burnetii/imunologia , Febre Q/veterinária , Doenças dos Ovinos/microbiologia , Animais , Portugal/epidemiologia , Febre Q/epidemiologia , Febre Q/microbiologia , Estudos Soroepidemiológicos , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/imunologia
17.
Transbound Emerg Dis ; 65(4): 972-975, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29799172

RESUMO

Q fever is a worldwide zoonotic infectious disease caused by Coxiella burnetii and sheep and goats are known to be the main reservoir for human infection. This study describes the epidemiological and laboratory findings of C. burnetii outbreaks affecting sheep and goat flocks and also provides the results of a prospective serosurvey in bulk tank milk samples to assess C. burnetii circulation in a population of sheep living in close contact to the human population in Central Portugal. In the epizooties, C. burnetii was identified in tissues of the resulting abortions by qPCR. As for the serological survey, 10.2% (95%CI: 4.5-19.2) of the 78 bulk tank milk samples collected in 2015 presented IgG antibodies against C. burnetii. The same farms were visited and sampled in 2016 and 25.6% (95%CI: 16.4-36.8) were positive. This steep increase in the number of anti-C. burnetii farms between the 2015 and 2016 collections showed to be statistically significant (p = 0.020) and is strongly suggestive of Q fever emergence in Central Portugal. Measures on animal health and on disease spread control to the human population should be considered.


Assuntos
Aborto Animal/epidemiologia , Coxiella burnetii/isolamento & purificação , Surtos de Doenças/veterinária , Doenças das Cabras/epidemiologia , Leite/virologia , Febre Q/epidemiologia , Doenças dos Ovinos/epidemiologia , Aborto Animal/virologia , Animais , Anticorpos Antibacterianos/sangue , Coxiella burnetii/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Doenças das Cabras/virologia , Cabras , Portugal/epidemiologia , Gravidez , Estudos Prospectivos , Febre Q/veterinária , Febre Q/virologia , Reação em Cadeia da Polimerase em Tempo Real , Ovinos , Doenças dos Ovinos/virologia , Inquéritos e Questionários , Zoonoses/epidemiologia
18.
Vector Borne Zoonotic Dis ; 16(1): 63-5, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26771815

RESUMO

Between November and December of 2014, a serosurvey was set up to evaluate the presence of Schmallenberg virus (SBV) antibodies in sheep of Portugal. Sera (n = 1068) were tested using an indirect enzyme linked immunosorbent assay (ID Screen(®) Schmallenberg virus indirect, IDvet Innovative Diagnostics, Montpellier, France). The estimated occurrence of immunogobulin G (IgG) antibodies against SBV in sheep of Portugal was 12.8% (95% confidence interval 11.0-15.0%). This is the first study reporting the presence of SBV antibodies in sheep of Portugal.


Assuntos
Anticorpos Antivirais/sangue , Infecções por Bunyaviridae/veterinária , Orthobunyavirus/genética , Doenças dos Ovinos/virologia , Animais , Infecções por Bunyaviridae/sangue , Infecções por Bunyaviridae/epidemiologia , Infecções por Bunyaviridae/virologia , Feminino , Imunoglobulina G/sangue , Portugal/epidemiologia , Estudos Soroepidemiológicos , Ovinos , Doenças dos Ovinos/sangue , Doenças dos Ovinos/epidemiologia
19.
Rio de Janeiro; IPEA; 2016. 41 p. graf.(Texto para Discussão / IPEA).
Monografia em Português | LILACS, ECOS | ID: biblio-992096

RESUMO

"Analisa a dins do índice desde 1999, e propôs-se um tradutor entre esses subitens e os conceitos da Classificação Nacional de Atividades Econômicas (CNAE), versão 2.0. Com base no referido tradutor, foi possível extrair dados da Pesquisa Anual de Serviços (PAS), da Pesquisa Nacional por Amostra de Domicílios (Pnad) e da Relação Anual das Informações Sociais (Rais) sobre a composição do valor da produção, das ocupações e dos rendimentos do trabalho dos vários setores de serviços que compõem o IPCA. Os dados analisados corroboram visões preexistentes sobre o tema e as estendem, propondo, em particular, que seja dada atenção a alguns poucos setores de serviços com taxas excepcionais de crescimento da produção e/ou do número de ocupados e rendimentos desses trabalhadores e que demandam políticas públicas específicas.


Assuntos
Inflação , Planos de Pré-Pagamento em Saúde , Serviços de Saúde , Brasil
20.
J. vasc. bras ; 12(4): 329-334, Oct-Dec/2013. graf
Artigo em Inglês | LILACS | ID: lil-699135

RESUMO

Aneurysms of the subclavian-axillary segment are rare, but when diagnosed they must be treated. This article describes two cases of aneurysms of the upper extremities, one in a subclavian artery and the other in an axillary artery. The first case was a 71-year-old male with a pulsating supraclavicular bulge on the right and muscle weakness in the ipsilateral extremity. Duplex scanning and arteriography confirmed the diagnosis of aneurysm of the right subclavian artery and the patient underwent aneurysmectomy and end-to-end anastomosis. The second case was a 24-year-old female patient, with no history of comorbidities, who presented with a pulsating mass in the right axillary region and paresthesia of the ipsilateral extremity. Duplex scanning and arteriography confirmed an aneurysm in the right axillary artery, which was successfully treated with aneurysmectomy and end-to-end anastomosis. Pathology findings showed that the first case was an atherosclerotic aneurysm and the second was a congenital aneurysm.


Os aneurismas do segmento subclávio-axilar são de ocorrência rara e, uma vez diagnosticados, devem ser tratados. Neste trabalho, relatamos dois casos de aneurismas de membros superiores, sendo um da artéria subclávia e outro da artéria axilar. No primeiro caso, o paciente de 71 anos, sexo masculino, apresentava abaulamento pulsátil supraclavicular direito associado à fraqueza muscular no membro ipsilateral. Foram realizados duplex scan e arteriografia, que confirmaram o diagnóstico de aneurisma da artéria subclávia direita, sendo o paciente submetido à aneurismectomia com anastomose término-terminal. No segundo caso, uma paciente de 24 anos, do sexo feminino, sem antecedentes mórbidos ou comorbidades, apresentava queixa de massa pulsátil na região axilar direita associada à parestesia no membro ipsilateral. O duplex scan e a arteriografia confirmaram o aneurisma da artéria axilar direita, que foi tratado com aneurismectomia e anastomose término-terminal com sucesso. O diagnóstico anátomo-clínico foi de aneurisma aterosclerótico no primeiro caso e, no segundo caso, de aneurisma congênito.


Assuntos
Humanos , Masculino , Feminino , Adulto Jovem , Idoso , Aneurisma/cirurgia , Aneurisma/congênito , Aneurisma/diagnóstico , Artéria Axilar/patologia , Artéria Subclávia/patologia , Angiografia/instrumentação
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